Hotspot

psbE-petL

LSC · 66721-67883 · 1163 bp

Back to Aralia

Pi 0.0117
MarkerSeek score 59.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1163
variable_sites
59
pi
0.0117
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GCCGCTAGTAGAAAACCGAA 50.0 50.0 57.9 58.0 1290-1314 bp 1.0 85.0
psbE-petL_p2 CAACCCGCAATGAATAGGGA AAGCCGCTAGTAGAAAACCG 50.0 50.0 57.9 58.0 1292-1316 bp 1.0 85.0
psbE-petL_p3 ACGAATAACCAACCCGCAAT GCCGCTAGTAGAAAACCGAA 45.0 50.0 58.2 58.0 1299-1323 bp 1.0 84.0
psbE-petL_p4 ACGAATAACCAACCCGCAAT AAGCCGCTAGTAGAAAACCG 45.0 50.0 58.2 58.0 1301-1325 bp 1.0 84.0
psbE-petL_p5 TGCTGACGAATAACCAACCC GCCGCTAGTAGAAAACCGAA 50.0 50.0 58.2 58.0 1304-1328 bp 1.0 83.9