Hotspot

psaI-ycf4

LSC · 61762-62168 · 407 bp

Back to Aralia

Pi 0.016
MarkerSeek score 59.40
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
LSC
length_bp
407
variable_sites
28
pi
0.016
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 GCAATGGCAATGGCTTCTTT TTGTTTTTCGAGACCCCGTT 45.0 45.0 57.9 58.0 514-519 bp 1.0 84.4
psaI-ycf4_p2 TAGGCCTAGTATTTCCGGCA TTGTTTTTCGAGACCCCGTT 50.0 45.0 57.9 58.0 531-536 bp 1.0 84.3
psaI-ycf4_p3 AGTATTTCCGGCAATGGCAA TTGTTTTTCGAGACCCCGTT 45.0 45.0 58.2 58.0 524-529 bp 1.0 83.9
psaI-ycf4_p4 GCAATGGCAATGGCTTCTTT ATAACGGCCCAGCAGAAATT 45.0 45.0 57.9 57.9 536-541 bp 1.0 83.4
psaI-ycf4_p5 TAGGCCTAGTATTTCCGGCA ATAACGGCCCAGCAGAAATT 50.0 45.0 57.9 57.9 553-558 bp 1.0 83.3