Hotspot

ndhC-trnV-UAC

LSC · 52648-53619 · 972 bp

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Pi 0.0143
MarkerSeek score 58.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
972
variable_sites
64
pi
0.0143
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT TTTAGGTGCCAAACAGACCC 50.0 50.0 57.9 58.0 1543-1583 bp 1.0 84.5
ndhC-trnV-UAC_p2 TGGTTCAGGCATTTGCTCAT TTTAGGTGCCAAACAGACCC 45.0 50.0 58.1 58.0 2151-2193 bp 1.0 83.5
ndhC-trnV-UAC_p3 TGGTTCAGGCATTTGCTCAT ATGAAAGAGCCCAATGCACA 45.0 45.0 58.1 58.1 1801-1842 bp 1.0 83.1
ndhC-trnV-UAC_p4 TGGTTCAGGCATTTGCTCAT GACAAAGGGTTTGGTCCGAT 45.0 50.0 58.1 58.1 2183-2225 bp 1.0 82.8
ndhC-trnV-UAC_p5 TGGTTCAGGCATTTGCTCAT CCCCACCGTTGATTTGAGAT 45.0 50.0 58.1 57.9 2134-2176 bp 0.947368 80.2