Hotspot

ycf3-trnS-GGA

LSC · 46620-47467 · 848 bp

Back to Aralia

Pi 0.0134
MarkerSeek score 66.00
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
848
variable_sites
49
pi
0.0134
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTGGGAA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.3 58.1 784-1034 bp 1.0 73.5
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTGGGA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.3 58.1 785-1035 bp 1.0 73.5
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTGGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.3 58.2 805-1055 bp 1.0 72.9
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTGGGA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.3 58.2 806-1056 bp 1.0 72.9
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTGGGAA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.3 57.8 755-1005 bp 1.0 72.8