Hotspot

trnH-GUG-psbA

LSC · 82-491 · 410 bp

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Pi 0.0346
MarkerSeek score 68.20
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
410
variable_sites
65
pi
0.0346
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TCGAAGCTCCATCTACAAATGG 55.0 45.5 58.1 58.2 441-474 bp 1.0 73.3
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TCGAAGCTCCATCTACAAATGG 50.0 45.5 57.9 58.2 455-488 bp 1.0 73.3
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TCGAAGCTCCATCTACAAATGG 50.0 45.5 57.9 58.2 454-487 bp 1.0 73.3
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TCGAAGCTCCATCTACAAATGG 50.0 45.5 57.9 58.2 456-489 bp 1.0 73.3
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CGAAGCTCCATCTACAAATGG 55.0 47.6 58.1 56.8 440-473 bp 1.0 73.1