Hotspot

rpoC2

LSC · 15991-20127 · 4137 bp

Back to Aragoa

Pi 0.000725
MarkerSeek score 54.30
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
LSC
length_bp
4137
variable_sites
3
pi
0.000725
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 CGTTGAGTTGACCCATTCGA GGATTCCGGATTGCTCTCTG 50.0 55.0 58.2 58.1 206 bp 1.0 77.7
rpoC2_p2 TCGTTGAGTTGACCCATTCG GGATTCCGGATTGCTCTCTG 50.0 55.0 58.2 58.1 207 bp 1.0 77.7
rpoC2_p3 CGTTGAGTTGACCCATTCGA AGGAATGCTCCATCGGATTC 50.0 50.0 58.2 57.4 220 bp 1.0 74.2
rpoC2_p4 TCGTTGAGTTGACCCATTCG AGGAATGCTCCATCGGATTC 50.0 50.0 58.2 57.4 221 bp 1.0 74.2
rpoC2_p5 CGTTGAGTTGACCCATTCGA TCGGAAAAAGGAATGCTCCA 50.0 45.0 58.2 57.4 228 bp 1.0 74.1