Hotspot

petB

LSC · 72769-74144 · 1376 bp

Back to Aragoa

Pi 0.000731
MarkerSeek score 59.70
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1376
variable_sites
1
pi
0.000731
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 TTGGTAGTTCGATCGTGGAA GAAAGATCCAAATCCATGTCT 45.0 38.1 56.6 53.6 1533-1541 bp 1.0 40.5
petB_p2 TTTGGTAGTTCGATCGTGGA GAAAGATCCAAATCCATGTCT 45.0 38.1 56.6 53.6 1534-1542 bp 1.0 40.5
petB_p3 TTGGTAGTTCGATCGTGGAA GAAAGATCCAAATCCATGTCTT 45.0 36.4 56.6 54.4 1533-1541 bp 1.0 40.5
petB_p4 TTTGGTAGTTCGATCGTGGA GAAAGATCCAAATCCATGTCTT 45.0 36.4 56.6 54.4 1534-1542 bp 1.0 40.5
petB_p5 TGGTAGTTCGATCGTGGAAT GAAAGATCCAAATCCATGTCT 45.0 38.1 56.4 53.6 1532-1540 bp 1.0 40.5