Hotspot

ndhF-rpl32

SSC · 109483-110215 · 733 bp

Back to Aragoa

Pi 0.00273
MarkerSeek score 50.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
733
variable_sites
2
pi
0.00273
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGTTGCTGTCGGAAAAAGA GCCCCTTTCTTTTCCAAAAA 45.0 40.0 58.0 54.8 906 bp 1.0 54.4
ndhF-rpl32_p2 TGGTTGCTGTCGGAAAAAGA GCCCCTTTCTTTTCCAAAAAT 45.0 38.1 58.0 55.4 906 bp 1.0 51.2
ndhF-rpl32_p3 TGGTTGCTGTCGGAAAAAGA GCCCCTTTCTTTTCCAAAAATT 45.0 36.4 58.0 56.2 906 bp 1.0 49.4
ndhF-rpl32_p4 TTGGTTGCTGTCGGAAAAAG GCCCCTTTCTTTTCCAAAAA 45.0 40.0 57.1 54.8 907 bp 1.0 47.9
ndhF-rpl32_p5 TGGTTGCTGTCGGAAAAAGA GCCCCTTTCTTTTCCAAAAATTT 45.0 34.8 58.0 56.9 906 bp 1.0 47.0