Hotspot

rpl22-rps19

LSC · 81518-81575 · 58 bp

Back to Aragoa

Pi 0.0172
MarkerSeek score 62.40
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
58
variable_sites
1
pi
0.0172
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TGACGACCTAAAGCGTATACT TTCGAGGACATGCAAAAAGC 42.9 45.0 56.3 57.3 167 bp 1.0 53.3
rpl22-rps19_p2 TGACGACCTAAAGCGTATACTT TTCGAGGACATGCAAAAAGC 40.9 45.0 57.0 57.3 167 bp 1.0 51.1
rpl22-rps19_p3 AGTGGGTTAAATCTTGGTACCA TTCGAGGACATGCAAAAAGC 40.9 45.0 57.0 57.3 122 bp 1.0 51.0
rpl22-rps19_p4 TGACGACCTAAAGCGTATACT TTTCGAGGACATGCAAAAAGC 42.9 42.9 56.3 58.0 168 bp 1.0 50.9
rpl22-rps19_p5 CGACCTAAAGCGTATACTTCTGT TTCGAGGACATGCAAAAAGC 43.5 45.0 58.1 57.3 164 bp 1.0 50.2