Hotspot

accD-psaI

LSC · 56834-57583 · 750 bp

Back to Aragoa

Pi 0.00533
MarkerSeek score 61.80
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
750
variable_sites
4
pi
0.00533
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCAGAAGGGCTTATTCGACC TTGCAAGTGCCGGAAATACT 55.0 45.0 58.0 58.1 929 bp 1.0 79.2
accD-psaI_p2 TAAGCTCCACGCCTTCTTTC TTGCAAGTGCCGGAAATACT 50.0 45.0 57.9 58.1 863 bp 1.0 78.8
accD-psaI_p3 CTCCACGCCTTCTTTCCTTT TTGCAAGTGCCGGAAATACT 50.0 45.0 58.1 58.1 859 bp 1.0 78.8
accD-psaI_p4 CCAGAAGGGCTTATTCGACC AAAGAAGCCATTGCAAGTGC 55.0 45.0 58.0 57.8 939 bp 1.0 78.7
accD-psaI_p5 TAAGCTCCACGCCTTCTTTC AAAGAAGCCATTGCAAGTGC 50.0 45.0 57.9 57.8 873 bp 1.0 78.3