Hotspot

ndhC-trnV-UAC

LSC · 48461-49500 · 1040 bp

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Pi 0
MarkerSeek score 60.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1040
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTCCGGCCCTTTACTAATCG CCGAGAAAGTCTACGGTTCG 55.0 55.0 57.8 58.1 1172-1193 bp 1.0 79.4
ndhC-trnV-UAC_p2 CTCCGGCCCTTTACTAATCG GTTCGAGTCCGTATAGCCCT 55.0 55.0 57.8 58.7 1157-1178 bp 1.0 74.8
ndhC-trnV-UAC_p3 CTCCGGCCCTTTACTAATCG CGAGAAAGTCTACGGTTCGA 55.0 50.0 57.8 57.1 1171-1192 bp 1.0 73.1
ndhC-trnV-UAC_p4 CTCCGGCCCTTTACTAATCG TTCGAGTCCGTATAGCCCTA 55.0 50.0 57.8 57.1 1156-1177 bp 1.0 72.7
ndhC-trnV-UAC_p5 CTCCGGCCCTTTACTAATCG AAAGTCTACGGTTCGAGTCC 55.0 50.0 57.8 57.0 1167-1188 bp 1.0 72.2