Hotspot

psbM-trnD-GUC

LSC · 28640-29134 · 495 bp

Back to Aragoa

Pi 0.00202
MarkerSeek score 51.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
495
variable_sites
1
pi
0.00202
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGCGGTAGGAACTAGAATGA TCAATTGGTTAGAGCACCGC 47.6 50.0 58.0 58.6 619 bp 1.0 67.5
psbM-trnD-GUC_p2 AGAAAGGCGGTAGGAACTAGA TCAATTGGTTAGAGCACCGC 47.6 50.0 57.9 58.6 623 bp 1.0 66.9
psbM-trnD-GUC_p3 AGTAGAAAGGCGGTAGGAACT TCAATTGGTTAGAGCACCGC 47.6 50.0 58.2 58.6 626 bp 1.0 66.3
psbM-trnD-GUC_p4 AGTAGAAAGGCGGTAGGAAC TCAATTGGTTAGAGCACCGC 50.0 50.0 56.7 58.6 626 bp 1.0 65.0
psbM-trnD-GUC_p5 GTAGAAAGGCGGTAGGAACT TCAATTGGTTAGAGCACCGC 50.0 50.0 56.7 58.6 625 bp 1.0 65.0