Hotspot

rps16-trnQ-UUG

LSC · 5921-6603 · 683 bp

Back to Aragoa

Pi 0.003
MarkerSeek score 62.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
683
variable_sites
2
pi
0.003
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 740-756 bp 1.0 80.2
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 751-767 bp 1.0 79.7
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AAAATTCAAATGGGGCGTGG 45.0 45.0 58.1 57.5 798-814 bp 1.0 76.9
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC AAAATTCAAATGGGGCGTGG 50.0 45.0 58.2 57.5 809-825 bp 1.0 76.4
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 744-760 bp 1.0 75.5