Hotspot

ycf1

SSC · 125762-131029 · 5268 bp

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Pi 0.00652

MarkerSeek score 42.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5268
variable_sites: 136
pi: 0.00652
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCTAGAACTTCCGCCCTGT	GAGCACATTCTCCATAGGGC	50.0	55.0	58.1	58.1	1156 bp	0.875	75.3
ycf1_p2	ATCTAGAACTTCCGCCCTGT	AGGGCCCTCTTATCTCTTCC	50.0	55.0	58.1	57.9	1141 bp	0.875	75.3
ycf1_p3	ATCTAGAACTTCCGCCCTGT	GGGCCCTCTTATCTCTTCCT	50.0	55.0	58.1	57.9	1140 bp	0.875	75.3
ycf1_p4	ATCTAGAACTTCCGCCCTGT	TATGCAAGAAGGGACCGAGA	50.0	50.0	58.1	58.1	1105 bp	0.875	75.1
ycf1_p5	ATCTAGAACTTCCGCCCTGT	CGAGCTCGGATTATGCAAGA	50.0	50.0	58.1	57.9	1116 bp	0.875	75.1