Hotspot

rpl32-trnL-UAG

SSC · 114660-115515 · 856 bp

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Pi 0.0197

MarkerSeek score 67.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 856
variable_sites: 65
pi: 0.0197
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGAGAGGTTTTTGGACAGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	844-996 bp	1.0	88.6
rpl32-trnL-UAG_p2	GGAGAGGTTTTTGGACAGCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	837-989 bp	1.0	88.2
rpl32-trnL-UAG_p3	GCGCAATCAATTTCTACGGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	806-958 bp	1.0	88.2
rpl32-trnL-UAG_p4	GGAGAGGTTTTTGGACAGCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	843-995 bp	1.0	88.1
rpl32-trnL-UAG_p5	GGAGAGGTTTTTGGACAGCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	842-994 bp	1.0	88.1