Hotspot

psbE-petL

LSC · 64999-66353 · 1355 bp

Back to Arachis

Pi 0.00865

MarkerSeek score 49.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1355
variable_sites: 45
pi: 0.00865
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAGCAAAAGAACGTTCTCCG	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.7	58.0	1409-1430 bp	1.0	81.7
psbE-petL_p2	CAGCAAAAGAACGTTCTCCG	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.7	58.0	1411-1432 bp	1.0	81.6
psbE-petL_p3	CAAAAGAACGTTCTCCGGTG	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.3	58.0	1406-1427 bp	1.0	78.8
psbE-petL_p4	CAAAAGAACGTTCTCCGGTG	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.3	58.0	1408-1429 bp	1.0	78.7
psbE-petL_p5	AGCAAAAGAACGTTCTCCGG	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.8	58.0	1408-1429 bp	1.0	77.9