Hotspot

atpI-atpH

LSC · 47877-49250 · 1374 bp

Back to Arachis

Pi 0.00782

MarkerSeek score 49.80

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1374
variable_sites: 37
pi: 0.00782
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TACAAGCGGTATTCAAGCCC	AGCAGCAATCAGTGGATTCA	50.0	45.0	58.0	57.5	1423-1480 bp	1.0	81.0
atpI-atpH_p2	TACAAGCGGTATTCAAGCCC	CAGCAGCAATCAGTGGATTC	50.0	50.0	58.0	57.2	1424-1481 bp	1.0	78.2
atpI-atpH_p3	CAAGCGGTATTCAAGCCCTT	AGCAGCAATCAGTGGATTCA	50.0	45.0	58.5	57.5	1421-1478 bp	1.0	76.9
atpI-atpH_p4	TACAAGCGGTATTCAAGCCC	AGCAGCAATCAGTGGATTCAT	50.0	42.9	58.0	58.0	1423-1480 bp	1.0	76.9
atpI-atpH_p5	TACAAGCGGTATTCAAGCCC	GCAGCAGCAATCAGTGGATT	50.0	50.0	58.0	59.2	1425-1482 bp	1.0	75.5