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psbM-petN

LSC · 32771-33874 · 1104 bp

Back to Arachis

Pi 0.00689

MarkerSeek score 53.50

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1104
variable_sites: 34
pi: 0.00689
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TCATTCTAGTTCCCACTGCC	TCTTGCTTGGGCTGCTTTAA	50.0	45.0	56.9	58.0	1234-1250 bp	1.0	75.2
psbM-petN_p2	CATTCTAGTTCCCACTGCCT	TCTTGCTTGGGCTGCTTTAA	50.0	45.0	56.9	58.0	1233-1249 bp	1.0	75.1
psbM-petN_p3	TCATTCTAGTTCCCACTGCC	ATCTTGCTTGGGCTGCTTTA	50.0	45.0	56.9	57.8	1235-1251 bp	1.0	73.5
psbM-petN_p4	TCATTCTAGTTCCCACTGCC	TAAATCTTGCTTGGGCTGCT	50.0	45.0	56.9	57.8	1238-1254 bp	1.0	73.4
psbM-petN_p5	CATTCTAGTTCCCACTGCCT	ATCTTGCTTGGGCTGCTTTA	50.0	45.0	56.9	57.8	1234-1250 bp	1.0	73.4