Hotspot

psbD-trnT-GGU

LSC · 29035-30568 · 1534 bp

Back to Arachis

Pi 0.00834

MarkerSeek score 59.30

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1534
variable_sites: 51
pi: 0.00834
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CACAAAAACGAAACGGTCCC	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.2	58.3	1519-1672 bp	1.0	82.9
psbD-trnT-GGU_p2	CCACAAAAACGAAACGGTCC	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.2	58.3	1520-1673 bp	1.0	82.8
psbD-trnT-GGU_p3	CCCACAAAAACGAAACGGTC	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.2	58.3	1521-1674 bp	1.0	82.8
psbD-trnT-GGU_p4	ACAAAAACGAAACGGTCCCT	GGCGTAAGTCATCGGTTCAA	45.0	50.0	58.2	58.3	1518-1671 bp	1.0	82.2
psbD-trnT-GGU_p5	AACCCACAAAAACGAAACGG	GGCGTAAGTCATCGGTTCAA	45.0	50.0	57.7	58.3	1523-1676 bp	1.0	82.0