Hotspot

trnS-UGA-psbC

LSC · 26342-26603 · 262 bp

Back to Arachis

Pi 0.0295

MarkerSeek score 66.30

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 262
variable_sites: 18
pi: 0.0295
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GTTGGAGAGATGGCTGAGTG	TAGGTCATTTATGGCACGCG	55.0	50.0	58.0	58.4	458-464 bp	1.0	81.1
trnS-UGA-psbC_p2	GAGAGATGGCTGAGTGGTTG	TAGGTCATTTATGGCACGCG	55.0	50.0	58.0	58.4	454-460 bp	1.0	81.0
trnS-UGA-psbC_p3	ATAGCCCCGGTCTTGAAAAC	TAGGTCATTTATGGCACGCG	50.0	50.0	57.9	58.4	434-440 bp	1.0	80.4
trnS-UGA-psbC_p4	GGGTTCGAATCCCTCTCTCT	TAGGTCATTTATGGCACGCG	55.0	50.0	58.2	58.4	386-392 bp	1.0	80.2
trnS-UGA-psbC_p5	TTTGGTTGGAGAGATGGCTG	TAGGTCATTTATGGCACGCG	50.0	50.0	57.8	58.4	462-468 bp	1.0	79.6