Hotspot

rps16

LSC · 5140-6239 · 1100 bp

Pi 0.0123

MarkerSeek score 54.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1100
variable_sites: 49
pi: 0.0123
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CATGTGTGCGTCAGGGATAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	2150-2171 bp	1.0	84.9
rps16_p2	CATGTGTGCGTCAGGGATAA	AAAAATCGACATGGGGCGTA	50.0	45.0	58.0	57.9	2209-2230 bp	1.0	84.4
rps16_p3	CATGTGTGCGTCAGGGATAA	TAAAAATCGACATGGGGCGT	50.0	45.0	58.0	57.9	2210-2231 bp	1.0	84.4
rps16_p4	AAATCCAGTCATGTGTGCGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	2159-2180 bp	1.0	84.2
rps16_p5	AAATCCAGTCATGTGTGCGT	AAAAATCGACATGGGGCGTA	45.0	45.0	58.1	57.9	2218-2239 bp	1.0	83.7