Hotspot

rpl32-trnL

SSC · 112381-113263 · 883 bp

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Pi 0.00942
MarkerSeek score 49.90
Primer available yes
Label rpl32-trnL

Feature Metadata

label_name
rpl32-trnL
feature_type
igs
region
SSC
length_bp
883
variable_sites
33
pi
0.00942
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_p1 AATGAGCACTCTTGTACGGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.6 57.9 1253-1281 bp 0.875 75.0
rpl32-trnL_p2 AATGAGCACTCTTGTACGGG TAACCACTGGGACTGGAGAG 50.0 55.0 57.6 58.1 1850-1877 bp 0.875 74.7
rpl32-trnL_p3 ATGAGCACTCTTGTACGGGA TAACCACTGGGACTGGAGAG 50.0 55.0 58.4 58.1 1849-1876 bp 0.875 74.3
rpl32-trnL_p4 TGAGCACTCTTGTACGGGAT TAACCACTGGGACTGGAGAG 50.0 55.0 58.4 58.1 1848-1875 bp 0.875 74.3
rpl32-trnL_p5 AATGAGCACTCTTGTACGGG AACAAAGCGCCATAGCCTAA 50.0 45.0 57.6 57.9 1894-1921 bp 0.875 74.2