Hotspot

trnP-psaJ

LSC · 65071-65434 · 364 bp

Back to Arabis

Pi 0.0175
MarkerSeek score 49.00
Primer available yes
Label trnP-psaJ

Feature Metadata

label_name
trnP-psaJ
feature_type
igs
region
LSC
length_bp
364
variable_sites
17
pi
0.0175
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.94
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-psaJ_p1 CTACCAAGCTGCGCTACATC CGGTGCTACGGAAAGATATGT 55.0 47.6 58.8 57.9 401-423 bp 1.0 72.3
trnP-psaJ_p2 TACCAAGCTGCGCTACATC CGGTGCTACGGAAAGATATGT 52.6 47.6 57.6 57.9 400-422 bp 1.0 67.7
trnP-psaJ_p3 CTACCAAGCTGCGCTACAT CGGTGCTACGGAAAGATATGT 52.6 47.6 57.6 57.9 401-423 bp 1.0 67.7
trnP-psaJ_p4 CTACCAAGCTGCGCTACATC CGGTGCTACGGAAAGATATG 55.0 50.0 58.8 56.1 401-423 bp 1.0 66.1
trnP-psaJ_p5 TACCAAGCTGCGCTACATC CGGTGCTACGGAAAGATATG 52.6 50.0 57.6 56.1 400-422 bp 1.0 61.5