Hotspot

psbE-petL

LSC · 63290-64206 · 917 bp

Back to Arabis

Pi 0.00788
MarkerSeek score 60.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
917
variable_sites
26
pi
0.00788
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ATAACCAGCCCGCAATGAAT AGCTGCTAGTAGAAAACCGA 45.0 45.0 57.9 56.3 1062-1071 bp 1.0 68.1
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.8 56.3 980-989 bp 1.0 67.7
psbE-petL_p3 AATAACCAGCCCGCAATGAA AGCTGCTAGTAGAAAACCGA 45.0 45.0 58.2 56.3 1063-1072 bp 1.0 67.5
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC AGCTGCTAGTAGAAAACCGA 55.0 45.0 58.2 56.3 975-984 bp 1.0 67.3
psbE-petL_p5 ATAACCAGCCCGCAATGAAT AGCTGCTAGTAGAAAACCGAA 45.0 42.9 57.9 57.0 1062-1071 bp 1.0 66.2