Hotspot

petA-psbJ

LSC · 61532-62504 · 973 bp

Back to Arabis

Pi 0.0137
MarkerSeek score 60.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
973
variable_sites
40
pi
0.0137
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC GCTGGTATTCCTGTGATCGG 50.0 55.0 58.0 58.1 1134-1167 bp 1.0 84.5
petA-psbJ_p2 CATTACGTGTCCAAGGCCTT GCTGGTATTCCTGTGATCGG 50.0 55.0 58.2 58.1 1131-1164 bp 1.0 83.4
petA-psbJ_p3 TTACGTGTCCAAGGCCTTTT GCTGGTATTCCTGTGATCGG 45.0 55.0 57.6 58.1 1129-1162 bp 1.0 82.0
petA-psbJ_p4 ACGTGTCCAAGGCCTTTTAT GCTGGTATTCCTGTGATCGG 45.0 55.0 57.4 58.1 1127-1160 bp 1.0 80.2
petA-psbJ_p5 ATTACGTGTCCAAGGCCTTT GCTGGTATTCCTGTGATCGG 45.0 55.0 57.4 58.1 1130-1163 bp 1.0 80.2