Hotspot

rbcL-accD

LSC · 55388-56123 · 736 bp

Back to Arabis

Pi 0.00948
MarkerSeek score 51.10
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
736
variable_sites
25
pi
0.00948
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 TGCAATGCACGCTGTTATTG TAACTAGAACGCTCACCCCA 45.0 50.0 58.0 58.1 1455-1465 bp 1.0 82.0
rbcL-accD_p2 TCATGGTATGCACTTCCGTG TAACTAGAACGCTCACCCCA 50.0 50.0 58.0 58.1 1422-1432 bp 1.0 82.0
rbcL-accD_p3 TTGAATGCTACTGCGGGTAC TAACTAGAACGCTCACCCCA 50.0 50.0 58.0 58.1 1622-1632 bp 1.0 81.7
rbcL-accD_p4 TTGAATGCTACTGCGGGTAC ATAGTGCGGATCGTTGTCAC 50.0 50.0 58.0 58.1 1856-1870 bp 0.875 74.9
rbcL-accD_p5 TTGAATGCTACTGCGGGTAC CATAGTGCGGATCGTTGTCA 50.0 50.0 58.0 58.1 1857-1871 bp 0.875 74.9