Hotspot

ndhC-trnV

LSC · 49430-50140 · 711 bp

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Pi 0.00809
MarkerSeek score 59.30
Primer available yes
Label ndhC-trnV

Feature Metadata

label_name
ndhC-trnV
feature_type
igs
region
LSC
length_bp
711
variable_sites
21
pi
0.00809
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV_p1 TGCCAAAACAGGAATAGCACT GTTCGAGTCCGTATAGCCCT 42.9 55.0 58.1 58.7 782-804 bp 1.0 71.0
ndhC-trnV_p2 TGCCAAAACAGGAATAGCACT TTCGAGTCCGTATAGCCCTA 42.9 50.0 58.1 57.1 781-803 bp 1.0 69.0
ndhC-trnV_p3 TGCCAAAACAGGAATAGCAC GTTCGAGTCCGTATAGCCCT 45.0 55.0 56.6 58.7 782-804 bp 1.0 68.8
ndhC-trnV_p4 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCAG 42.9 47.6 58.1 58.1 810-832 bp 1.0 67.8
ndhC-trnV_p5 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCA 42.9 45.0 58.1 56.9 810-832 bp 1.0 67.5