Hotspot

trnT-trnL

LSC · 45481-46324 · 844 bp

Back to Arabis

Pi 0.00725
MarkerSeek score 60.50
Primer available yes
Label trnT-trnL

Feature Metadata

label_name
trnT-trnL
feature_type
igs
region
LSC
length_bp
844
variable_sites
22
pi
0.00725
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-trnL_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCAATTCC 55.0 55.0 57.9 58.2 878-906 bp 1.0 83.1
trnT-trnL_p2 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 881-909 bp 1.0 82.3
trnT-trnL_p3 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 910-938 bp 1.0 82.0
trnT-trnL_p4 GGAATCGAACCGATGACCAT AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 913-941 bp 1.0 81.3
trnT-trnL_p5 GCGATGCTCTAACCTCTGAG TAAGTCCGTAGCGTCTACCA 55.0 50.0 57.9 57.6 883-911 bp 1.0 81.0