Hotspot

psaA-ycf3

LSC · 41053-41781 · 729 bp

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Pi 0.00447
MarkerSeek score 57.10
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
729
variable_sites
12
pi
0.00447
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT ATTGGTTGACGATCACGAGG 50.0 50.0 57.9 58.0 838-861 bp 1.0 80.9
psaA-ycf3_p2 AGAAATGACCGGGTTTAGCC ATTGGTTGACGATCACGAGG 50.0 50.0 57.9 58.0 855-878 bp 1.0 80.8
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT AACGCTTACGCCTGGTAATT 50.0 45.0 57.9 57.9 873-896 bp 1.0 80.1
psaA-ycf3_p4 AGAAATGACCGGGTTTAGCC AACGCTTACGCCTGGTAATT 50.0 45.0 57.9 57.9 890-913 bp 1.0 80.0
psaA-ycf3_p5 GCCCATTCCTCGAAAGAAGT ACAGGCTATAACGCTTACGC 50.0 50.0 57.9 58.2 882-905 bp 1.0 79.7