Hotspot

psbE-ORF31

LSC · 64357-65738 · 1382 bp

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Pi 0.00889

MarkerSeek score 63.50

Primer available yes

Label psbE-ORF31

Feature Metadata

label_name: psbE-ORF31
feature_type: igs
region: LSC
length_bp: 1382
variable_sites: 72
pi: 0.00889
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-ORF31_p1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	57.9	56.3	1533-1544 bp	1.0	70.2
psbE-ORF31_p2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1451-1462 bp	1.0	69.8
psbE-ORF31_p3	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1534-1545 bp	1.0	69.5
psbE-ORF31_p4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	1446-1457 bp	1.0	69.5
psbE-ORF31_p5	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGAA	45.0	42.9	57.9	57.0	1533-1544 bp	1.0	68.3