Hotspot

petA-psbJ

LSC · 62555-63571 · 1017 bp

Back to Arabidopsis

Pi 0.00555

MarkerSeek score 61.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1017
variable_sites: 38
pi: 0.00555
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTACGTGTCCAAGGCCTTTT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.6	58.1	1107-1212 bp	1.0	82.8
petA-psbJ_p2	TACGTGTCCAAGGCCTTTTG	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.4	58.1	1106-1211 bp	1.0	82.5
petA-psbJ_p3	TGTCCAAGGCCTTTTGTTCT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.5	58.1	1102-1207 bp	1.0	82.1
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.0	58.1	1113-1217 bp	0.916667	80.6
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.2	58.1	1110-1214 bp	0.916667	79.5