Hotspot

rbcL-accD

LSC · 56356-57037 · 682 bp

Back to Arabidopsis

Pi 0.00981

MarkerSeek score 63.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 682
variable_sites: 35
pi: 0.00981
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGCAAGTGGAGTCCTGAAC	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.0	57.9	833-849 bp	1.0	86.7
rbcL-accD_p2	TTGCAAGTGGAGTCCTGAAC	GCTCGCCTCTGTATTCCAAT	50.0	50.0	58.0	57.8	831-847 bp	1.0	85.3
rbcL-accD_p3	CTTGCAAGTGGAGTCCTGAA	TAGCTCGCCTCTGTATTCCA	50.0	50.0	57.7	57.9	834-850 bp	1.0	84.9
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	TAGCTCGCCTCTGTATTCCA	55.0	50.0	57.7	57.9	824-840 bp	1.0	84.6
rbcL-accD_p5	TTGCAAGTGGAGTCCTGAAC	TGCTTTACTTAGCTCGCCTC	50.0	50.0	58.0	57.7	842-858 bp	0.875	77.0