Hotspot

atpB-rbcL

LSC · 54107-54915 · 809 bp

Back to Arabidopsis

Pi 0.00649

MarkerSeek score 66.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 809
variable_sites: 33
pi: 0.00649
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	943-985 bp	1.0	78.2
atpB-rbcL_p2	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	942-984 bp	1.0	77.9
atpB-rbcL_p3	TCCAGTACCGGACCAATGAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	941-983 bp	1.0	77.6
atpB-rbcL_p4	CGAAACCTCTGGATCCGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	888-927 bp	0.916667	73.3
atpB-rbcL_p5	CGAAACCTCTGGATCCGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	887-926 bp	0.916667	73.0