Hotspot

psbG-ndhC

LSC · 49880-49940 · 61 bp

Back to Arabidopsis

Pi 0.0143

MarkerSeek score 61.30

Primer available yes

Label psbG-ndhC

Feature Metadata

label_name: psbG-ndhC
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 5
pi: 0.0143
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbG-ndhC_p1	GGCTGGAAAGTCTTGACCAA	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	170-201 bp	1.0	86.1
psbG-ndhC_p2	GGCTGGAAAGTCTTGACCAA	CTGGGGGCATCTGCTTTTAT	50.0	50.0	58.0	57.9	237-268 bp	1.0	86.0
psbG-ndhC_p3	GGCTGGAAAGTCTTGACCAA	AAAGGAGCATTGGAGTGGTC	50.0	50.0	58.0	57.8	162-193 bp	1.0	85.2
psbG-ndhC_p4	GGCTGGAAAGTCTTGACCAA	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.0	57.8	258-289 bp	1.0	84.8
psbG-ndhC_p5	GGCTGGAAAGTCTTGACCAA	GAGTTTCGATGTACTGGGGG	50.0	55.0	58.0	57.7	250-281 bp	1.0	84.0