Hotspot

trnL-trnF

LSC · 47477-47964 · 488 bp

Back to Arabidopsis

Pi 0.0166

MarkerSeek score 62.00

Primer available yes

Label trnL-trnF

Feature Metadata

label_name: trnL-trnF
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 35
pi: 0.0166
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnF_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	402-903 bp	1.0	83.8
trnL-trnF_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	399-900 bp	1.0	80.5
trnL-trnF_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	391-892 bp	1.0	79.9
trnL-trnF_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	364-865 bp	1.0	79.7
trnL-trnF_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	374-875 bp	1.0	79.5