Hotspot

ycf9-trnG

LSC · 35889-36383 · 495 bp

Back to Arabidopsis

Pi 0.00941

MarkerSeek score 62.50

Primer available yes

Label ycf9-trnG

Feature Metadata

label_name: ycf9-trnG
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 29
pi: 0.00941
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf9-trnG_p1	TCCTGATGGTTGGTCGAGTA	CGGATAGCGGGAATCGAAC	50.0	57.9	57.8	58.2	653-744 bp	1.0	75.9
ycf9-trnG_p2	TCCTGATGGTTGGTCGAGTA	GATAGCGGGAATCGAACCC	50.0	57.9	57.8	57.8	651-742 bp	1.0	75.7
ycf9-trnG_p3	TCCTGATGGTTGGTCGAGTA	GGATAGCGGGAATCGAACC	50.0	57.9	57.8	57.8	652-743 bp	1.0	75.7
ycf9-trnG_p4	CCGTTGTATTTGCGTCTCCT	CGGATAGCGGGAATCGAAC	50.0	57.9	58.3	58.2	669-760 bp	1.0	75.3
ycf9-trnG_p5	CCGTTGTATTTGCGTCTCCT	GATAGCGGGAATCGAACCC	50.0	57.9	58.3	57.8	667-758 bp	1.0	75.1