Hotspot

trnK-rps16

LSC · 4308-4993 · 686 bp

Back to Arabidopsis

Pi 0.0121
MarkerSeek score 59.30
Primer available yes
Label trnK-rps16

Feature Metadata

label_name
trnK-rps16
feature_type
igs
region
LSC
length_bp
686
variable_sites
52
pi
0.0121
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-rps16_p1 CCTTTCAGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 1027-1071 bp 0.916667 83.4
trnK-rps16_p2 CCTTTCAGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 1028-1072 bp 0.916667 83.4
trnK-rps16_p3 TCAGGATCAGTCGTGGTCTT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 1023-1067 bp 0.916667 83.0
trnK-rps16_p4 TCAGGATCAGTCGTGGTCTT TCCCAATGAGCCGTTTATCG 50.0 50.0 58.1 58.1 1024-1068 bp 0.916667 83.0
trnK-rps16_p5 TTCAGGATCAGTCGTGGTCT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 1024-1068 bp 0.916667 83.0