Hotspot

trnK-matK

LSC · 1722-2024 · 303 bp

Back to Arabidopsis

Pi 0.00777

MarkerSeek score 45.90

Primer available yes

Label trnK-matK

Feature Metadata

label_name: trnK-matK
feature_type: igs
region: LSC
length_bp: 303
variable_sites: 14
pi: 0.00777
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-matK_p1	ACTCGAACCCGGAACTAGTC	TCGAGTGCGAATTTGGTATTTG	55.0	40.9	58.8	57.7	386-393 bp	1.0	59.2
trnK-matK_p2	GACTCGAACCCGGAACTAGT	TCGAGTGCGAATTTGGTATTTG	55.0	40.9	58.8	57.7	387-394 bp	1.0	59.2
trnK-matK_p3	ACTCGAACCCGGAACTAGTC	TCGAGTGCGAATTTGGTATT	55.0	40.0	58.8	55.5	386-393 bp	1.0	57.7
trnK-matK_p4	TACGATCCATAGTGGGTTGC	TCGAGTGCGAATTTGGTATTTG	50.0	40.9	57.1	57.7	411-418 bp	0.958333	57.3
trnK-matK_p5	CGATCCATAGTGGGTTGCC	TCGAGTGCGAATTTGGTATTTG	57.9	40.9	58.0	57.7	409-416 bp	0.958333	56.5