Hotspot

ycf1

SSC · 124508-129886 · 5379 bp

Back to Arabidella

Pi 0.0146

MarkerSeek score 50.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5379
variable_sites: 208
pi: 0.0146
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTTTACGCCTTTGGGAACG	AGGGGAGGATCTGGACAAAA	50.0	50.0	58.0	57.9	1242 bp	1.0	83.1
ycf1_p2	GAACGAATTCCAGGCTCCAT	TTTTGCATGGGATCTCTCGG	50.0	50.0	58.0	58.0	439 bp	0.714286	73.5
ycf1_p3	GTTTTACGCCTTTGGGAACG	TTTTGCATGGGATCTCTCGG	50.0	50.0	58.0	58.0	622 bp	0.714286	72.5
ycf1_p4	TCTTCGTTTTCGCCTTCCAA	TTTTGCATGGGATCTCTCGG	45.0	50.0	58.0	58.0	409 bp	0.428571	61.1
ycf1_p5	TTCTTCGTTTTCGCCTTCCA	TTTTGCATGGGATCTCTCGG	45.0	50.0	58.0	58.0	410 bp	0.428571	61.1