Hotspot

rpl20-rps12

LSC · 69098-69854 · 757 bp

Back to Arabidella

Pi 0.0143

MarkerSeek score 63.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 28
pi: 0.0143
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	870-887 bp	1.0	81.4
rpl20-rps12_p2	TTTTGTTCTACGCCTCCGAG	ACAGCCAATCCGAAATGTCA	50.0	45.0	57.9	57.8	884-901 bp	1.0	81.0
rpl20-rps12_p3	TCCGAGCTATATATCCGCGT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	814-831 bp	1.0	80.9
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	875-892 bp	1.0	80.8
rpl20-rps12_p5	TTTTGTTCTACGCCTCCGAG	ACAAGACAGCCAATCCGAAA	50.0	45.0	57.9	57.7	889-906 bp	1.0	80.4