Hotspot

psaI-ycf4

LSC · 59476-59877 · 402 bp

Back to Arabidella

Pi 0.0118

MarkerSeek score 63.50

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 14
pi: 0.0118
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GGCAATTGCAATGGCTTCTT	TTGTTTTTCGAGACCCCGTT	45.0	45.0	57.9	58.0	515-522 bp	1.0	81.3
psaI-ycf4_p2	TAGTCTTTCCGGCAATTGCA	TTGTTTTTCGAGACCCCGTT	45.0	45.0	57.8	58.0	525-532 bp	1.0	80.5
psaI-ycf4_p3	GGCAATTGCAATGGCTTCTT	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.9	57.9	539-546 bp	1.0	80.4
psaI-ycf4_p4	AGTAGGCCTAGTCTTTCCGG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.2	58.0	533-540 bp	1.0	80.3
psaI-ycf4_p5	GCCTAGTCTTTCCGGCAATT	TTGTTTTTCGAGACCCCGTT	50.0	45.0	58.2	58.0	528-535 bp	1.0	80.1