Hotspot

trnH-psbA

LSC · 77-359 · 283 bp

Back to Arabidella

Pi 0.0563
MarkerSeek score 75.00
Primer available yes
Label trnH-psbA

Feature Metadata

label_name
trnH-psbA
feature_type
igs
region
LSC
length_bp
283
variable_sites
36
pi
0.0563
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-psbA_p1 CTGCCTTAATCCACTTGGCT GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.9 58.1 330-347 bp 1.0 89.1
trnH-psbA_p2 CTGCCTTAATCCACTTGGCT CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 345-362 bp 1.0 88.9
trnH-psbA_p3 ACTGCCTTAATCCACTTGGC GCTGTTGAGGCTCCATCTAC 50.0 55.0 58.2 58.1 331-348 bp 1.0 88.9
trnH-psbA_p4 CTGCCTTAATCCACTTGGCT TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 348-365 bp 1.0 88.8
trnH-psbA_p5 ACTGCCTTAATCCACTTGGC CCTCTAGACCTAGCTGCTGT 50.0 55.0 58.2 57.9 346-363 bp 1.0 88.7