Hotspot

ndhF-rpl32

SSC · 119498-120348 · 851 bp

Back to Aquilegia

Pi 0.00905

MarkerSeek score 60.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 851
variable_sites: 63
pi: 0.00905
unique_haplotype_count: 49
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ATAGGAACTGGAAGGGGGAC	AACTTTTCGAACCCCCTTCC	55.0	50.0	58.1	58.0	887-1112 bp	0.971429	87.4
ndhF-rpl32_p2	GGAGAAGACCCACCCCTATT	AACTTTTCGAACCCCCTTCC	55.0	50.0	58.1	58.0	910-1135 bp	0.971429	87.4
ndhF-rpl32_p3	GAACAAGGAGAAGACCCACC	AACTTTTCGAACCCCCTTCC	55.0	50.0	57.8	58.0	916-1141 bp	0.957143	86.1
ndhF-rpl32_p4	GGAACAAGGAGAAGACCCAC	AACTTTTCGAACCCCCTTCC	55.0	50.0	57.8	58.0	917-1142 bp	0.957143	86.1
ndhF-rpl32_p5	AAGACCCACCCCTATTGCTA	AACTTTTCGAACCCCCTTCC	50.0	50.0	57.7	58.0	906-1131 bp	0.971429	86.0