Hotspot

ycf1-ndhF

SSC · 117231-118009 · 779 bp

Back to Aquilegia

Pi 0.0135

MarkerSeek score 64.00

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 779
variable_sites: 62
pi: 0.0135
unique_haplotype_count: 65
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACGAAGGGGACTAAACAGGA	CACCCCTTGCTTGTTTTTGG	50.0	50.0	58.0	58.0	1268-1398 bp	1.0	87.3
ycf1-ndhF_p2	ACGAAGGGGACTAAACAGGA	CCACCCCTTGCTTGTTTTTG	50.0	50.0	58.0	58.0	1269-1399 bp	1.0	87.3
ycf1-ndhF_p3	ACAGGAACAAGAGGGATCCA	CACCCCTTGCTTGTTTTTGG	50.0	50.0	58.0	58.0	1254-1384 bp	1.0	87.2
ycf1-ndhF_p4	ACGAAGGGGACTAAACAGGA	GGCTCTTATGGGCGGTTTAA	50.0	50.0	58.0	58.0	1351-1481 bp	1.0	86.8
ycf1-ndhF_p5	ACAGGAACAAGAGGGATCCA	GGCTCTTATGGGCGGTTTAA	50.0	50.0	58.0	58.0	1337-1467 bp	1.0	86.7