Hotspot

rpl16-rps3

LSC · 87230-87388 · 159 bp

Back to Aquilegia

Pi 0.0116

MarkerSeek score 52.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 14
pi: 0.0116
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAGAGCGTATCCACCGAAAC	TCGAGATATCCATGGGGCAT	50.0	50.0	58.0	58.0	330-336 bp	1.0	83.5
rpl16-rps3_p2	GCCTCGATAAGATACCCCCT	TCGAGATATCCATGGGGCAT	55.0	50.0	58.1	58.0	300-306 bp	1.0	83.4
rpl16-rps3_p3	AAGAGCGTATCCACCGAAAC	CCTACAAACCATTCGAGCGA	50.0	50.0	58.0	58.0	375-381 bp	1.0	83.3
rpl16-rps3_p4	GCCTCGATAAGATACCCCCT	CCTACAAACCATTCGAGCGA	55.0	50.0	58.1	58.0	345-351 bp	1.0	83.2
rpl16-rps3_p5	AGCGTATCCACCGAAACAAA	CCTACAAACCATTCGAGCGA	45.0	50.0	57.8	58.0	372-378 bp	1.0	82.0