Hotspot

psbT-psbN

LSC · 78572-78666 · 95 bp

Back to Aquilegia

Pi 0.00915

MarkerSeek score 53.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 95
variable_sites: 8
pi: 0.00915
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGCCTAGGGTTCCAACTAAA	GATCCATTCGAGGAACACGG	50.0	55.0	57.2	58.4	147-152 bp	1.0	76.8
psbT-psbN_p2	CGCCTAGGGTTCCAACTAAA	GGGTACGCCTTATATACCGC	50.0	55.0	57.2	57.7	195-200 bp	1.0	76.4
psbT-psbN_p3	CGCCTAGGGTTCCAACTAAA	GTTTTACCGGGTACGCCTTA	50.0	50.0	57.2	57.6	203-208 bp	1.0	75.5
psbT-psbN_p4	CGCCTAGGGTTCCAACTAAA	TGTCAGTTTTACCGGGTACG	50.0	50.0	57.2	57.6	208-213 bp	1.0	74.9
psbT-psbN_p5	CGCCTAGGGTTCCAACTAAA	CGGGTACGCCTTATATACCG	50.0	55.0	57.2	57.5	196-201 bp	1.0	74.3