Hotspot

trnF-GAA-ndhJ

LSC · 50164-50743 · 580 bp

Back to Aquilegia

Pi 0.00382

MarkerSeek score 53.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 580
variable_sites: 17
pi: 0.00382
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TTTGATGCCCGAAAGTTGGA	50.0	45.0	58.1	58.0	686-703 bp	1.0	84.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	679-696 bp	1.0	84.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	678-695 bp	1.0	84.0
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TATGATAATCACCCCCGCCT	50.0	50.0	58.1	58.0	715-732 bp	1.0	83.8
trnF-GAA-ndhJ_p5	CTAACATAGACCCCAGCCCT	TTTGATGCCCGAAAGTTGGA	55.0	45.0	58.2	58.0	767-784 bp	1.0	82.6