Hotspot

trnT-GGU-psbD

LSC · 33447-34784 · 1338 bp

Back to Aquilegia

Pi 0.00361

MarkerSeek score 53.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1338
variable_sites: 25
pi: 0.00361
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	TGTCTTCACGTTGGCTCATT	GGGAAAGAGCAATAGACCGG	45.0	55.0	57.7	58.0	1525-1584 bp	1.0	81.9
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	GGGAAAGAGCAATAGACCGG	50.0	55.0	58.3	58.0	1454-1513 bp	1.0	81.9
trnT-GGU-psbD_p3	CCCCTTTAACTCAGCGGTAG	GGGAAAGAGCAATAGACCGG	55.0	55.0	57.7	58.0	1490-1549 bp	1.0	81.5
trnT-GGU-psbD_p4	TGTCTTCACGTTGGCTCATT	ACAAAAACGAAACGGTCCCT	45.0	45.0	57.7	58.2	1497-1556 bp	1.0	80.3
trnT-GGU-psbD_p5	TGTCTTCACGTTGGCTCATT	GACCGGACCAACCTACAAAA	45.0	50.0	57.7	57.7	1511-1570 bp	1.0	80.2