Hotspot

rps16-trnQ-UUG

LSC · 6511-7638 · 1128 bp

Back to Aquilegia

Pi 0.00369
MarkerSeek score 57.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1128
variable_sites
39
pi
0.00369
unique_haplotype_count
36
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AGAACCCACAACCGATCATG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1217-1239 bp 1.0 83.8
rps16-trnQ-UUG_p2 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1212-1234 bp 1.0 83.8
rps16-trnQ-UUG_p3 AACGAGCACCTCAGAATTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1260-1282 bp 1.0 83.7
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1191-1213 bp 1.0 83.7
rps16-trnQ-UUG_p5 AACCCACAACCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1215-1237 bp 1.0 83.3