Hotspot

trnK-UUU-rps16

LSC · 4733-5374 · 642 bp

Back to Aquilegia

Pi 0.00739

MarkerSeek score 64.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 642
variable_sites: 47
pi: 0.00739
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	ATTTCCTTGAAAAAGGCGCG	50.0	45.0	58.2	57.9	671-791 bp	1.0	87.1
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	ATTTCCTTGAAAAAGGCGCG	50.0	45.0	58.2	57.9	670-790 bp	1.0	87.1
trnK-UUU-rps16_p3	TCTACCAACGGTATGGACGA	ATTTCCTTGAAAAAGGCGCG	50.0	45.0	57.9	57.9	736-856 bp	1.0	87.0
trnK-UUU-rps16_p4	TCTAGCCGCACTTAAAAGCC	ATTTCCTTGAAAAAGGCGCG	50.0	45.0	58.3	57.9	685-805 bp	1.0	86.2
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	ATTTCCTTGAAAAAGGCGCG	50.0	45.0	58.3	57.9	680-800 bp	1.0	86.1